Gene mapping and identification of candidate genes controlling . . . Integrated transcriptomic and metabolomic profiling during grain filling in both yellow and white varieties pinpointed SiPSY1 and SiCCD1 as key genes controlling carotenoid accumulation Notably, the SNP (G A) at 364 bp and the Indel (29 bp insertion) at 856 bp within the SiPSY1 promoter predominantly contributed to the variance in promoter
Gene mapping and identification of candidate genes controlling . . . These variations markedly affected SiPSY1 expression levels, ultimately determining the phenotypic difference between yellow and white kernels These findings provide crucial genetic insights for the molecular mechanisms involved in carotenoid metabolism and lay a solid foundation for millet color breeding in foxtail millet
Understanding carotenoid biosynthetic pathway control points using . . . LC and mass spectrometry-based metabolomics can be used to analyze many metabolites of the carotenoid biosynthetic pathway This chapter describes methodologies for large-scale metabolite analysis and genetic mapping to identify genes that regulate content and composition of carotenoids and apocarotenoids
Gene mapping and identification of candidate genes controlling . . . This study elucidated the genetic and molecular mechanisms governing carotenoid accumulation in foxtail millet kernels, revealing SiPSY1 and SiCCD1 as key regulators of color variation A SNP (G A) at 364 bp and a 29 bp Indel at 856 bp in the SiPSY1 promoter reduced its expression, thereby determining the phenotypic distinction between yellow
Gene mapping and identification of candidate genes controlling . . . Herein, the molecular mechanisms governing carotenoid accumulation in the kernel of foxtail millet were investigated by an exhaustive methodology encompassing Genome-Wide Association Study (GWAS), Bulk Segregant Analysis sequencing (BSA-seq), and integrated transcriptomic and metabolomic analyses
Foxtail millet: nutritional and eating quality, and prospects for . . . Gene mapping and identification of candidate genes controlling carotenoid accumulation of yellow kernels in foxtail millet: Wang Junjie, Ma Qi, Zhang Yuyang, Duan Qian, Zhen Xiaoxi, Zhang Yaoyuan, Li Hongying, Han Yuanhuai, Zhang Bin, BMC Plant Biology 2025 25(1):null doi:10 1186 s12870-025-06585-9 4
An intronic SNP in the Carotenoid Cleavage Dioxygenase 1 (CsCCD1 . . . In this study, we employed map-based cloning to identify a Carotenoid Cleavage Dioxygenase 1 (CsCCD1) as a key genetic factor influencing yellow flesh in cucumbers A causal single nucleotide polymorphism (SNP) in the eighth intron of CsCCD1 led to aberrant splicing, resulting in a truncated transcript
Identification of genomic regions and candidate genes underlying . . . Collectively, understanding the genetic basis of carotenoid pigments and identifying genes underpinning carotenoid accumulation via a bulked-segregant analysis-based sequencing (BSA-seq) approach provide new insights for exploring future molecular breeding efforts to produce soybean cultivars with high carotenoid content
Advances in the Discovery and Engineering of Gene Targets for . . . The identification and manipulation of gene targets that influence the accumulation of the desired products is a crucial challenge in the construction and metabolic regulation of recombinant strains In this review, we provide an overview of the carotenoid biosynthetic pathway, followed by a summary of the methodologies employed in the